Title
Atomic Force Microscopy Study of Lrp Protein-DNA Interaction at the Solid-liquid Interface.
Document Type
Article
Publication Date
2001
Abstract
A phase variation control mechanism involving Pap B, Pap I, catabolite activator protein (CAP), leucine responsive regulatory protein (Lrp) and deoxyadenosine methylase (Dam) regulate the expression of pyelonephritis-associated pilus (Pap). This study centers on elucidating the role of Lrp in the transcription of Pap pilus. Specifically, we will measure the translocation of Lrp bound to plasmid DNA of Escherichia coli induced by Pap I. A hypothesis is that three Lrp dimers interact with the Pap I and translocate 102 base pairs from a GATC-II to a GATC-I sequence. In preparation for this measurement, we have obtained Atomic Force Microscopy (AFM) images that show Lrp bound to a particular binding site on DNA in buffer solution.
Recommended Citation
Toyoda, Shunsuke; Duong, Lin; Stella, Karen; Hernday*, Aaron; and Low*, David, "Atomic Force Microscopy Study of Lrp Protein-DNA Interaction at the Solid-liquid Interface." (2001). URC Student Scholarship.
http://scholar.oxy.edu/urc_student/385
Advisor
Eileen M. Spain
Department
chem
Support
Howard Hughes Medical Institute
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