Hannemania bufonis, n. sp. is described from larvae taken off the red spotted toad, Bufo punctatus Baird and Girard. The type locality is Whitewater Canyon, Riverside Co., California, and it also is known from Arizona, western Texas, southern Utah and Sonora, Mexico. Closely similar is Hannemania hylae (Ewing) redescribed from topotypes taken off the type host, Hyla cadaverina Cope (originally listed as Hyla arenicolor) from Cottonwood Creek, San Diego Co., California. Additional locality and host records include larvae from Hyla arenicolor, taken in Arizona, western Texas, southern Utah, and northern Sonora, Mexico, and off Hyla cadaverina from Baja California Norte, Mexico.