Biochemistry
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Death and All Its Friends: The Role of Programmed Death-1 in T-cell Exhaustion During Chronic Viral Infection
(2013-01-01)Programmed Death-1 (PD-1) is a CD-28 family inhibitory immune receptor that is located on Tc cells, particularly during chronic infection. Its role in regulating the immune response is crucial because it ensures a middle ... -
Characterization of an Alpha Glucosidase from the Bacterial Predator Bdellovibrio Bacteriovorus
(2003-01-01)An unusual alpha glucosidase gene from the bacterial predator <i style="mso-bidi-font-style:normal">Bdellovibrio bacteriovorus has been isolated and identified and is currently being characterized. Substrate specificity ... -
FRET-based Assay for Small Ubiquitin-like Modifier (SUMO)-specific Protease Inhibitors Screening
(2009-01-01)This summer I continued my project from last summer of screening for small ubiquitin-like modifier (SUMO)-specific protease inhibitors. Small ubiquitin-like modifier (SUMO) becomes covalently conjugated to target proteins ... -
FRET-based Assay for Small Ubiquitin-like Modifier (SUMO)-specific Protease Inhibitors Screening
(2008-01-01)Small ubiquitin-like modifiers (SUMOs) become covalently conjugated to target proteins in a process called sumoylation. Desumoylation involves removal of the SUMO-moiety from target proteins. In higher eukaryotes, there ... -
Quantitative Analysis of the Autophagy-Lysosomal Pathway in Alzheimer's type Neurodegeneration
(2009-01-01)The proteotoxic peptide amyloid beta 42 (Ab42) is believed to play a causative role in Alzheimer?s disease. However, the mechanisms leading to the characteristic senile plaques and mass cell death are still unknown. The ... -
The Development and Characterization of Polyvalent Anti-Influenza Antivirals
(2012-07-01)The influenza virus infects host cells via the collective interaction (polyvalence) between many hemagglutinin (HA) sites, which are glycoproteins found on the viral envelope, and sialic acid (SA) residues found on the ... -
Reciprocal Differentiation and Tissue-Specific Pathogenesis of Th1, Th2, and Th17 in Graft-versus-Host Disease
(2008-01-01)Graft-versus-Host-Disease (GVHD) is still the major obstacle for the widespread application of allogeneic hematopoietic cell transplantation (HCT). Cytokines from activated and differentiated donor CD4<sup>+</sup> T cells ... -
Purification and Characterization of Tomato Plantocyanin Protein
(2006-01-01)Blue copper proteins were among the first proteins to be isolated and their dazzling intense blue color has attracted many scientists. Blue Copper Binding proteins (BCBs) have been purified and characterized in Archea, ... -
Identification of periplasmically regulated genes in Bdellovibrio bacteriovorus using an IVET approach.
(2001-01-01)Bdellovibrio bacteriovorus is a predatory bacterium with two separate life phases. In one, it searches for a suitable Gram negative bacterium to attack and invade. The second phase of life is spent after invasion, within ... -
Effect of Nucleotide Excision Repair on Methylglyoxal-Induced DNA Damage
(2008-01-01)Methylglyoxal (MG) is an endogenous mutagen, formed nonenzymatically from triose phosphates during glycolysis in eukaryotic cells. MG reacts with amino groups in proteins and DNA, forming advanced glycation end-products ... -
TR-FRET Assay for Sirtuin 1 (SIRT1) Inhibitor Screening
(2008-01-01)SIRT1 is an enzyme from the sirtuin family. Sirtuins are NAD<sup>+</sup>-dependent protein deacetylases. This enzyme family is responsible in pathways involved in apoptosis, cellular stress, and aging. Theories have been ... -
Optimizing the purification of wild type and mutant recombinant phospholipase D: An approach to producing a Corynebacterium pseudotuberculosis vaccine
(2012-07-01)The overall goal of the lab is to characterize the equine immune response to the disease, ‘pigeon fever’, caused by the gram-positive bacteria C. pseudotuberculosis. Mainly prevalent in warmer climates, but with increasing ... -
Analysis of Glucose-6-Phosphate Dehydrogenaseafter Purification usingGel Electrophoresis and Western Blotting
(2002-01-01)The matrix used in our work was a polyacrylamide gel of 5% concentration of the acrylamide monomer crosslinked to N,N'-methylene bisacrylamide (bisacrylamide). In order to estimate purity and molecular weight in electrophoresis ... -
Increasing the yield of Glucose-6-Phosphate Dehydrogenase.
(2001-01-01)Glucose-6-Phosphate Dehydrogenase (G6PD) is responsible for catalyzing the initial oxidative reaction- the conversion of Glucose-6-Phosphate (G6P) into 6-Phosphogluconolactone - in the Pentose Phosphate Pathway (PPP). It ... -
Structure Influencing Function: Using Glucose-6-Phosphate Dehydrogenase as a Model System for Protein-Protein Interactions
(2005-01-01)Glucose-6-phosphate dehydrogenase (G6PDH) catalyzes the first and rate-determining step in the Pentose Phosphate Pathway, a secondary pathway for glucose metabolism in animal cells. G6PDH oxidizes glucose-6-phosphate to ... -
Glucose-6-Phosphate Dehydrogenase Association Studies.
(2000-01-01)Glucose-6-Phosphate dehydrogenase (G6PD) catalyzes cellular biochemical oxidation-reduction reactions. Glucose-6-phosphate undergoes oxidation to 6-phosphogluconolactone while nicotinamide adenine dinucleotide phosphate ... -
Human Erythrocyte Glucose-6-Phosphate Dehydrogenase Association Studies
(2003-01-01)Glucose-6-phosphate dehydrogenase (G6PD) catalyzes the first step in the pentose phosphate pathway by converting glucose-6-phosphate to 6-phosphogluconolactone. During this rate-limiting step, nicotinamide adenine dinucleotide ... -
The Glucose-6-Phosphate Dehydrogenase Dimer Interface: Proteolytic Studies with NADP Analogues
(2008-01-01)Glucose-6-phosphate dehydrogenase (G6PDH) catalyzes the first and rate-limiting step of the pentose phosphate pathway, which oxidizes glucose to 6-Phosphoglucono-δ-lactone and reduces NADP to NADPH. G6PDH exists in ... -
Cancer and Glycation--Using Human Tissue Culture Model to Probe the Metabolic Effects of Glycation Flux
(2009-01-01)Understanding the growth of cancerous tumors versus typical epithelial cells is important in not only detection of cancer but alternative treatments and potential cures. With a greater understanding of tumor conditions, ...
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