Biochemistry
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Death and All Its Friends: The Role of Programmed Death-1 in T-cell Exhaustion During Chronic Viral Infection
(2013-01-01)Programmed Death-1 (PD-1) is a CD-28 family inhibitory immune receptor that is located on Tc cells, particularly during chronic infection. Its role in regulating the immune response is crucial because it ensures a middle ... -
FRET-based Assay for Small Ubiquitin-like Modifier (SUMO)-specific Protease Inhibitors Screening
(2009-01-01)This summer I continued my project from last summer of screening for small ubiquitin-like modifier (SUMO)-specific protease inhibitors. Small ubiquitin-like modifier (SUMO) becomes covalently conjugated to target proteins ... -
FRET-based Assay for Small Ubiquitin-like Modifier (SUMO)-specific Protease Inhibitors Screening
(2008-01-01)Small ubiquitin-like modifiers (SUMOs) become covalently conjugated to target proteins in a process called sumoylation. Desumoylation involves removal of the SUMO-moiety from target proteins. In higher eukaryotes, there ... -
Quantitative Analysis of the Autophagy-Lysosomal Pathway in Alzheimer's type Neurodegeneration
(2009-01-01)The proteotoxic peptide amyloid beta 42 (Ab42) is believed to play a causative role in Alzheimer?s disease. However, the mechanisms leading to the characteristic senile plaques and mass cell death are still unknown. The ... -
Characterization of an Alpha Glucosidase from the Bacterial Predator Bdellovibrio Bacteriovorus
(2003-01-01)An unusual alpha glucosidase gene from the bacterial predator <i style="mso-bidi-font-style:normal">Bdellovibrio bacteriovorus has been isolated and identified and is currently being characterized. Substrate specificity ... -
Effect of Nucleotide Excision Repair on Methylglyoxal-Induced DNA Damage
(2008-01-01)Methylglyoxal (MG) is an endogenous mutagen, formed nonenzymatically from triose phosphates during glycolysis in eukaryotic cells. MG reacts with amino groups in proteins and DNA, forming advanced glycation end-products ... -
TR-FRET Assay for Sirtuin 1 (SIRT1) Inhibitor Screening
(2008-01-01)SIRT1 is an enzyme from the sirtuin family. Sirtuins are NAD<sup>+</sup>-dependent protein deacetylases. This enzyme family is responsible in pathways involved in apoptosis, cellular stress, and aging. Theories have been ... -
Optimizing the purification of wild type and mutant recombinant phospholipase D: An approach to producing a Corynebacterium pseudotuberculosis vaccine
(2012-07-01)The overall goal of the lab is to characterize the equine immune response to the disease, ‘pigeon fever’, caused by the gram-positive bacteria C. pseudotuberculosis. Mainly prevalent in warmer climates, but with increasing ... -
Reciprocal Differentiation and Tissue-Specific Pathogenesis of Th1, Th2, and Th17 in Graft-versus-Host Disease
(2008-01-01)Graft-versus-Host-Disease (GVHD) is still the major obstacle for the widespread application of allogeneic hematopoietic cell transplantation (HCT). Cytokines from activated and differentiated donor CD4<sup>+</sup> T cells ... -
Purification and Characterization of Tomato Plantocyanin Protein
(2006-01-01)Blue copper proteins were among the first proteins to be isolated and their dazzling intense blue color has attracted many scientists. Blue Copper Binding proteins (BCBs) have been purified and characterized in Archea, ... -
Identification of periplasmically regulated genes in Bdellovibrio bacteriovorus using an IVET approach.
(2001-01-01)Bdellovibrio bacteriovorus is a predatory bacterium with two separate life phases. In one, it searches for a suitable Gram negative bacterium to attack and invade. The second phase of life is spent after invasion, within ... -
The Development and Characterization of Polyvalent Anti-Influenza Antivirals
(2012-07-01)The influenza virus infects host cells via the collective interaction (polyvalence) between many hemagglutinin (HA) sites, which are glycoproteins found on the viral envelope, and sialic acid (SA) residues found on the ... -
Metal Homeostasis: Intracellular Copper Trafficking and ALS Anh Dang, Brittany Taunton, Yumi Tomita
(2007-01-01)Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disease characterized by the quick devastation of nerve cells in the brain and spinal column. No cause or cure for ALS is currently known, but a subset of familial ... -
Analysis of Glucose-6-Phosphate Dehydrogenaseafter Purification usingGel Electrophoresis and Western Blotting
(2002-01-01)The matrix used in our work was a polyacrylamide gel of 5% concentration of the acrylamide monomer crosslinked to N,N'-methylene bisacrylamide (bisacrylamide). In order to estimate purity and molecular weight in electrophoresis ... -
The Purification and Characterization of Lily Chemocyanin and Arabidopsis Plantacyanin
(2006-01-01)Blue copper binding proteins are prevalent in all forms of life, yet most of them have not been functionally identified. These proteins have special folding characteristics, which allow them to bind a single copper atom, ... -
Glucose-6-Phosphate Dehydrogenase (G6PD) Association Studies
(2002-01-01)Glucose-6-Phosphate Dehydrogenase (G6PD) catalyzes the first step in the Pentose Phosphate Pathway by oxidizing glucose-6-phosphate (G6P) to 6-phosphogluconolactone. Simultaneously, nicotinamide adenine dinucleotide phosphate ... -
Characterization and Immunodetection of Human Glucose-6-Phosphate Dehydrogenase
(2003-01-01)In the process of gel electrophoresis and Western blotting, human glucose-6-phosphate dehydrogenase (G6PD) is separated from other proteins, transferred to a nitrocellulose membrane and further detected by the use of ... -
Chlamydomonas Multicopper Oxidase:An Intriguing Ortholog of Metazoan Ceruloplasmin
(2005-01-01)Multicopper blue oxidases are composed of three or six domains, one of which includes Ceruloplasmin, a domain found only in metazoa. Recent genetic complementation studies in prokaryotic Bacillus sp. SG-1, targeting genes ... -
Increasing the yield of Glucose-6-Phosphate Dehydrogenase.
(2001-01-01)Glucose-6-Phosphate Dehydrogenase (G6PD) is responsible for catalyzing the initial oxidative reaction- the conversion of Glucose-6-Phosphate (G6P) into 6-Phosphogluconolactone - in the Pentose Phosphate Pathway (PPP). It ...
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