• Login
    View Item 
    •   Oxy Scholar Home
    • Biology
    • Biology URC Student Scholarship
    • View Item
    •   Oxy Scholar Home
    • Biology
    • Biology URC Student Scholarship
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    The Differentiation and Transplantation of GABA Over-expressing Embryonic Stem Cells

    Thumbnail
    Author
    Geraets, Daniel
    Issue
    urc_student; urc_student
    Date
    2007-01-01 0:00
    Metadata
    Show full item record
    URI
    https://scholar.oxy.edu/handle/20.500.12711/447
    Abstract
    Embryonic stem cell (ES) derived neurons represent a potential source of material for cellular transplantation into the brain. The ability to introduce transgenes into ES, to select and characterize clonal lines, and to direct those lines into neural phenotypes, permits opportunities to develop cell-based therapeutic strategies for intractable neurological diseases, such as epilepsy. Previous studies have revealed a potential therapeutic effect of the inhibitory neurotransmitter GABA in animal models of epilepsy. In this study we have genetically engineered the mouse ES cell line ZHTc6 to regulatably over-express the GABA-synthesizing enzyme GAD using lipofection. Clonally selected cell lines were differentiated into predominantly neuronal phenotypes by change of growth medium composition. The amount of GABA produced by differentiated and undifferentiated cell lines was measured in vitro using HPLC. In a pilot study, the undifferentiated, GABA over-expressing cells were transplanted into the hippocampus of a mouse. Transplanted cells were characterized using the reporter molecule α-galactosidase. In future experiments, the potential for transplanted ES derived neurons to suppress epileptic seizures will be measured using telemetry analysis. We will also conduct additional experiments focusing on the differentiation of GABA over-producing stem cells into predominantly glial phenotypes, for potential higher GABA expression and increased survival. These studies should increase our understanding of the therapeutic potential of embryonic stem cells.
    Collections
    • Biology URC Student Scholarship

    Browse

    All of Oxy ScholarCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsJournal TitleJournal IssueThis CollectionBy Issue DateAuthorsTitlesSubjectsJournal TitleJournal Issue

    My Account

    LoginRegister

    DSpace software copyright © 2002-2021  DuraSpace
    Contact Us | Send Feedback
    DSpace Express is a service operated by 
    Atmire NV