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    Mobilizing various plasmids into Bdellovibrio bacteriovorus

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    Author
    Kim, Min
    Issue
    urc_student; urc_student
    Date
    2002-01-01 0:00
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    URI
    https://scholar.oxy.edu/handle/20.500.12711/468
    Abstract
    This project focuses on mobilizing various plasmids into wild-type Bdellovibrio. Although we have become extremely successful in moving numerous plasmids into host-independent Bdellovibrio, working with wild-type Bdellovibrio has proven to be a much more dificult task. With host independent Bdellovibrio, the bacterium grows on nutrient medium and are easily isolated. Wild-type Bdellovibrio allows the researcher to observe first hand the activities of the bacterium as it invades host cells. This semester I was able to mobilize the pUTTcRpLac-RFP plasmid into wild-type Bdellovibrio 109J. Future work on this project may include additional matings with wild-type Bdellovibrio and other E.coli plasmids. If the matings are successful, then the recumbinant Bdellovibrio can be observed as it goes through the different stages of its life cycle.
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