• Login
    View Item 
    •   Oxy Scholar Home
    • Biology
    • Biology URC Student Scholarship
    • View Item
    •   Oxy Scholar Home
    • Biology
    • Biology URC Student Scholarship
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Regulation of Immunoglobulin Gene Rearrangement by a Chimeric Mouse-human Transgene

    Thumbnail
    Author
    Pang, Jenn
    Issue
    urc_student; urc_student
    Date
    2000-01-01 0:00
    Metadata
    Show full item record
    URI
    https://scholar.oxy.edu/handle/20.500.12711/494
    Abstract
    Immunoglobulin (Ig) genes undergo DNA rearrangement (termed VDJ rearrangement) during B cell development to produce antibodies. The regulation of VDJ rearrangement during B cell development is highly regulated. Once the heavy chain VDJ gene segments are successfully rearranged, a heavy chain protein is made. Normally, this heavy chain signals the cell to stop heavy chain VDJ rearrangement and to start light chain VJ rearrangement. Our transgenic mice express the HUG g1 heavy chain transgene at high levels.This transgenic heavy chain inhibits endogenous heavy chain rearrangement, but unexpectedly does not induce light rearrangement. This is the first demonstration that the mechanism that regulating heavy chain rearrangement can be distinguished from that regulating light chain rearrangement.My project is to develop an <i style="mso-bidi-font-style:normal">in vitro assay for studying this differential signaling.This involves hybridomas that are HUG+m-, and therefore results in no light chain rearrangement. I have transfected a rearranged endogenous heavy chain into two different cell lines and antibiotic selection is underway. I will then assay for production and association of the light chain protein.
    Collections
    • Biology URC Student Scholarship

    Browse

    All of Oxy ScholarCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsJournal TitleJournal IssueThis CollectionBy Issue DateAuthorsTitlesSubjectsJournal TitleJournal Issue

    My Account

    LoginRegister

    DSpace software copyright © 2002-2021  DuraSpace
    Contact Us | Send Feedback
    DSpace Express is a service operated by 
    Atmire NV