• Login
    View Item 
    •   Oxy Scholar Home
    • Chemistry
    • Chemistry URC Student Scholarship
    • View Item
    •   Oxy Scholar Home
    • Chemistry
    • Chemistry URC Student Scholarship
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Examination of Groove Binding and Intercalation into DNA by Atomic Force Microscopy

    Thumbnail
    Author
    Ly, Elaine
    Issue
    urc_student; urc_student
    Date
    2004-01-01 0:00
    Metadata
    Show full item record
    URI
    https://scholar.oxy.edu/handle/20.500.12711/637
    Abstract
    Atomic force microscopy (AFM) has been used to view DNA shape, condensation, and protein binding at the nanometer scale. Intercalators such as ethidium bromide induce changes in the superhelical shape of DNA, producing an extension, unwinding, and stiffening of the DNA helix. Groove-binding agents induce little change in the length or shape of DNA in solution; however, on a surface, these same cations can cause condensation of the DNA. We have used Tapping Mode AFM to examine the binding of a family of Ru<sup>2+</sup> octahedral metal complexes to DNA. Based on solution measurements, some of these complexes are proposed to intercalate, others bind to the groove of DNA via electrostatics, and still others have multiple binding modes. By measuring the length, height, and shape of hundreds of plasmid molecules at a variety of Ru<sup>2+</sup> concentrations, we have observed that the nature of the metal ligands affects the mode of DNA binding, and have found that the metal complex-DNA interactions at a surface can be more complex than those in solution.
    Collections
    • Chemistry URC Student Scholarship

    Browse

    All of Oxy ScholarCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsJournal TitleJournal IssueThis CollectionBy Issue DateAuthorsTitlesSubjectsJournal TitleJournal Issue

    My Account

    LoginRegister

    DSpace software copyright © 2002-2021  DuraSpace
    Contact Us | Send Feedback
    DSpace Express is a service operated by 
    Atmire NV