• Login
    View Item 
    •   Oxy Scholar Home
    • Chemistry
    • Chemistry URC Student Scholarship
    • View Item
    •   Oxy Scholar Home
    • Chemistry
    • Chemistry URC Student Scholarship
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Investigations of Mammalian Cytochrome P450 2B4 in Surfactant Films James M. Gillan and Katharine D. Hagen

    Thumbnail
    Author
    Gillan, JamesM.; Hagen, Katharine D
    Issue
    urc_student; urc_student
    Date
    2005-01-01 0:00
    Metadata
    Show full item record
    URI
    https://scholar.oxy.edu/handle/20.500.12711/639
    Abstract
    We report thermodynamic properties of the drug-metabolizing human cytochrome P450 2B4 within surfactant films based on electrochemical methods. The protein was embedded in didodecyldimethylammonium bromide (DDAB) bilayer film on a basal plane graphite electrode. Cyclic voltammetry at 25 mV/s was conducted in 50 mM KPi 50 mM KCl pH 7.0 buffer anaerobically between 18.4-40.4 ?C. Analysis of the half-wave potential dependence on temperature revealed decreases in entropy and enthalpy upon heme reduction. These thermodynamic changes are coupled to the expulsion of the heme distal axial water ligand; this is indicative of hydrophobic collapse around the catalytic center, resulting in a more rigid and stable structure. Spectroscopic analysis of the protein in the film revealed the Fe<sup>III</sup> heme Soret band at 418 nm, identical to its value in solution. This confirms protein integrity within the film, thereby affirming the physiological relevance of our thermodynamic data.
    Collections
    • Chemistry URC Student Scholarship

    Browse

    All of Oxy ScholarCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsJournal TitleJournal IssueThis CollectionBy Issue DateAuthorsTitlesSubjectsJournal TitleJournal Issue

    My Account

    LoginRegister

    DSpace software copyright © 2002-2021  DuraSpace
    Contact Us | Send Feedback
    DSpace Express is a service operated by 
    Atmire NV