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    Enzymatic protein P450: The potential when using electrochemistry Kevo Hindoyan & Clarisse van der Feltz

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    Author
    Hindoyan, Kevo
    Issue
    urc_student; urc_student
    Date
    2007-01-01 0:00
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    URI
    https://scholar.oxy.edu/handle/20.500.12711/655
    Abstract
    P450 is a well studied protein found perfectly tuned to the needs of a variety of organisms. Still, we have yet to fully harness the knowledge gained due to P450?s complex functions in vivo. Electrochemistry allows us to bypass several of the safeguards built into the activation of P450. This summer?s project is to tune this technique to more accurately mimic the processes found in nature. When having obtained this goal, the potential industrial and pharmaceutical applications are numerous. Our methods include synthesis of tethers connecting the protein to the electrode surface and the mutation of the protein to increase or decrease the speed with which the electron?s are transferred (the ET rate).
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