Strategies for Addressing Microelectrode Arrays Based on Cu(I)-Mediated Ethynyl-Azide Coupling
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The research in Professor Hill's lab is centered around exploring the electrochemical properties of DNA. Using this powerful attribute of DNA, this lab has already detected the presence of TATA binding proteins on nano-molar concentrations using a DNA/electrode interface. Modified DNA binds to a conductive linker on the electrode surface, where an electrical current may be induced through the DNA. DNA conducts electricity very well when its base-pairs are perfectly matched up, but its long-range electron transfer ability is altered when base-pair mutation occurs, or transcription factors bind to it. This alteration essentially generates an electric signature of that specific transcription factor My goal in the Hill group will be to help assemble and test an effective method to profile multiple DNA-binding proteins using electrochemistry. In order to accomplish this, we will need a micro-array of electrodes capable of performing electrochemistry on the contents of a single cell. Additionally, this micro-array will need to be highly localized so that specific DNA sequences may be addressed to particular parts of the array. This will allow for multiple proteins to be accounted for on a single chip at very small volumes and concentrations. My work specifically will most likely be centered around the synthesis of organometallic molecules relevant to this technique, as well as electrochemical analysis of DNA assays.