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    A Study of Soluble Glucose Dehydrogenase Based upon Kinetic Studies of the Structural Analogs of PQQ. James Ewing, Leslie Thompson & Carolnn Yong

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    Author
    Ewing, James; Leslie
    Issue
    urc_student; urc_student
    Date
    1999-01-01 0:00
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    URI
    https://scholar.oxy.edu/handle/20.500.12711/715
    Abstract
    Soluble glucose dehydrogenase is a homodimeric enzyme found in various bacteria such as Acinetobacter calcoaceticus , requiring Ca<sup>2+</sup> and pyrroloquinolinequinone (PQQ) cofactors for full enzymatic activity. sGDH catalyses the oxidation of glucose and other aldose sugars. The mechanistic details have yet to be disclosed. Towards that goal, a specific series of 3 isomeric analogs (2-4) of PQQ are being synthesized from methoxyaniline derivatives in the laboratory. The synthesis includes the reaction of a diazonium salt with ethyl-2-methyacetoacetate to yield the corresponding hydrazones of ethyl pyruvate. Each hydrazone underwent acid catalyzed Fischer indole synthesis followed by reduction of the nitro group using H2/Pd/C in to the corresponding aminoindole. Michael addition with dimethyl trans 2-ketoglutaconate was performed to form the third pyridine ring, which was further oxidized into triesters. Hydrolysis of the oxidation product yields the 3 isomeric analogs of PQQ. These isomers differ in the positioning of the nitrogen atom on the ring system and will serve to define structural significance in terms of binding with a metal cation and reconstitution process with the apoprotein. Detailed analysis of the stability of each reconstituted enzyme as well as steady-state kinetics studies will shed light on the structural characteristics mandatory for enzymatic activity and active site characterization.
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