Abstract
1'-ethyne-1-ferrocenecarboxylic acid is a compound which will serve as a linker molecule between DNA and a gold micro array. Its unique electrochemical properties allow quantitative data to be collected regarding the electrical conductivities of many samples of DNA, and their respective binding proteins simultaneously. The original synthesis began by deprotonating ferrocene with t-butyl lithium, and reacting it with tributyltin chloride to create a mixture of mono- and bis-substituted stannyl ferrocene. These were separated on a silica column, and the bis-substituted product was reacted with Iodine to form 1,1'-diiodoferrocene. The following reaction, which attempted a nucleophilic attack on carbon dioxide, was hindered due to the presence of various tin derivatives. An alternate synthesis of 1,1'-diiodoferrocene, involving a halomecuriferrocene, yields the product without Tin derivatives.
