Mobilizing various plasmids into Bdellovibrio bacteriovorus
This project focuses on mobilizing various plasmids into wild-type Bdellovibrio. Although we have become extremely successful in moving numerous plasmids into host-independent Bdellovibrio, working with wild-type Bdellovibrio has proven to be a much more dificult task. With host independent Bdellovibrio, the bacterium grows on nutrient medium and are easily isolated. Wild-type Bdellovibrio allows the researcher to observe first hand the activities of the bacterium as it invades host cells. This semester I was able to mobilize the pUTTcRpLac-RFP plasmid into wild-type Bdellovibrio 109J. Future work on this project may include additional matings with wild-type Bdellovibrio and other E.coli plasmids. If the matings are successful, then the recumbinant Bdellovibrio can be observed as it goes through the different stages of its life cycle.
Kim, Min, " Mobilizing various plasmids into Bdellovibrio bacteriovorus" (2002). URC Student Scholarship.