Phase Behavior of Phospholipid Bilayers with Ethanol.
Differential scanning calorimetry (DSC) and fluorescence spectroscopy are useful techniques in determining the threshold value for the onset of interdigitation in distearoylphosphatidylcholine (DSPC) multilayers. In both DSPC and DSPC/2 mol% cholesterol systems, the threshold value was found to be 4% ethanol. DSC results showed the biphasic effect with the disappearance of the pretransition below the threshold value and shoulder peaks on cooling scans above the threshold value. 1,6-diphenyl-1,3,5-hexatriene (DPH) was used to detect the great decrease in intensity as an indicator for interdigitation. Cholesterol lowered and broadened the transition temperatures of the phosphatidylcholine. 2 mol% cholesterol did not have much effect in the induction of interdigitated phase in DSPC.
Ho, Shirleen, "Phase Behavior of Phospholipid Bilayers with Ethanol." (2000). URC Student Scholarship.
Ford Research Fellowship